Polysciences offers a wide variety of products specific for microbiological and bacterial detection applications. Microring
®
bacterial
detectors are simple, rapid test methods which utilize specific growth factors to identify different microorganisms.
Microring
®
XV
Cat. #24644
(50 rings/pkg)
Microring
®
XV is a paper ring used in Haemophilus identification. It comes complete with individual printed tips containing haemin
(X-factor) and nicotinamide adenine dinucleotide-NAD (V-factor) or X+V factor. This ring differentiates Haemophilus species on the
basis of growth requirements for X and V factors and can be identified by their growth patterns close to the tips. V-tip also contains
δ
-aminolaevulinic acid (ALA) for Porphyrin test. (The porphyrin test is best performed at the start when the ring is laid on the plate
prior to incubation. Using a loop take a sample of the test organism from a growing culture and spread lightly over the V-tip.
Non-haemin requiring species will cause an orange-red glow to be seen from the V-tip.)
Procedure
1. Spread sample of the test organism onto an agar plate of Tryptone Soy Agar or Columbia Agar (without blood).
2. Using sterile forceps, carefully place Microring
®
XV in the center of the plate. Press each tip gently to ensure contact with surface.
3. Incubate plate overnight at 37° C.
4. Following incubation, growth patterns around each tip are read and used to identify the organism.
Differentiation of Haemophilus species
Species
H. influenzae
H. aegypticus
H. parainfluenzae
H. haemolyticus
H. parahaemolyticus
Growth results at tip
XV
V
X
NG NG
G
NG NG
G
G
NG G
NG
NG
NG
G
G
G
Growth factor
requirement
XV
XV
V
XV
V
XV = Haemin and NAD
Porphyrin
synthesis (ALA)
-
-
+
-
+
NG = No growth
Haemolysis on
horse blood*
-
-
-
+
+
G = Zone of growth
X = Haemin V = NAD and
δ
-aminolaevulinic acid (ALA)
* Haemolysis recorded from primary culture.
Microring
®
XV
continues - pg 2
Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information
given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since
conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice
any process in violation of any law or any government regulation.
Rings maintain a shelf life of 24 months from manufacture date.
Microring
®
AN
Cat. #24645
(50 rings/pkg)
Microring
®
AN is a filter paper ring used for the identification of Non-sporing Anaerobes. It has 6 tips which are impregnated with a
range of antibiotics for the identification of species of anaerobic Gram-negative bacilli and can distinguish Gram-positive cocci from
Gram-negative cocci. Gram-negative anaerobic rods are found in more than half of clinical specimens containing anaerobes. Although
difficult to perform precise identifications, in practice most groups can be presumptively identified from their antibiotic susceptibility
profile, which is the basis of the Microring
®
AN test scheme.
Procedure
1. Spread sample of test organism onto a plate containing anaerobic agar.
2. Using sterile forceps, carefully place Microring
®
AN in the center of the plate.
Press each tip gently to ensure contact with surface.
3. Incubate for 24-48 hours at 35-37° C under anaerobic conditions.
4. Following incubation, growth patterns around each tip are read and used
to identify the organism. Zone diameters of 15mm or greater are considerd
susceptible, while those less than 15mm are regarded as resistant.
Code
E
RP
CO
PG
K
VA
Color
Red
Dark red
White
Pink
Salmon
Blue
Antibiotic
Erythromycin
Rifampicin
Colistin
Penicillin G
Kanamycin
Vancomycin
Amount
60ug
15ug
10ug
2 units
1000ug
5ug
Identification Table
Species
B. Fragilis
Prevotella
melaninogenicus
Prevotella oralis
B. ureolyticus
E RP CO PG K VA
S
S
S
S
S
S
S
S
R
V
S
S
R
S
S
S
R
R
R
S
R
R
R
R
Aesculin
hydrolysis
+
–
+
–
Growth
in bile
+
–
–
–
Indole
+
–
–
–
Oxidase positive.
Black pigmented colonies with brick
red fluorescence.
Other details
E = Erythromycin, RP = Rifampicin, CO = Colistin, PG = Penicillin, K = Kanamycin, VA = Vancomycin
S = Sensitive, R = Resistant
Microring
®
AN
continues - pg 3
Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information
given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since
conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice
any process in violation of any law or any government regulation.
E = Erythromycin, RP = Rifampicin, CO = Colistin, PG = Penicillin, K = Kanamycin, VA = Vancomycin
Microring
®
AN (GIFU METHOD)
Recently a new identification scheme has been developed in Japan for use with Microring
®
AN. Prepare bacterial suspension as
above, but adjust turbidity to between 1 and 3 MacFarland. This ensures adequate visible growth within 48hours. Using this inoculum,
resistance and sensitivity are measured as follows:
Size of Zone (mm)
Interpretation
< 10
_
Resistant (R)
11-14
Intermediate (I)
> 15
_
Sensitive (S)
Species
B. fragilis
Prevotella melaninogenicus
Prevotella intermedia
Other pigmented prevotella
Prevotella oralis
Non-pigmented Prevotella
Porphyromonas
B. ureolyticus
F. nucleatum
F. varium
Gram-neg. cocci
Campylobacter
B. wadsworthia
Desulfovibrio
C. clostridioforme
C. symbiosum
Anaerbiospirillum
D. pneumonsintes
S. wadsorthensis
L. buccalis
C. ochracea
E
V
S
S
S
S
S
S
S
R
R
I/R
S
R
S
V
S/I
R
I
S
R
S
RP
S
S
S
S
S
S
S
I
S
R
S
R
R
R
R
S
S
I
S
S
S
CO
R
V
S
S
S
S
R
S
V
S
S
S
S
R
R
R
S
R
S
S
R
PG
R
S
V
S
S
R
S
I
S
S
R
R
R
R
R
R
R
R
R
R
I
K
R
R
R
R
R
R
R
S
S
S
V
S
S
S
S
S
S
S
S
S
R
VA
R
R
R
R
R
R
V
R
R
R
R
R
R
R
S
S
R
R
R
R
R
E = Erythromycin
RP = Rifampicin
CO = Colistin
PG = Penicillin
K = Kanamycin
VA = Vancomycin
S = Sensitive
R = Resistant
V = Variable
Storage & Handling
Store Microring
®
AN at 2-8°C
Rings maintain a shelf life of 12 months from manufacture date.
Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information
given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since
conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice
any process in violation of any law or any government regulation.
AC is a filter paper ring used for the identification of Gram-positive anaerobic cocci and some microaerophilic cocci. It has 3
individual printed tips containing various antimicrobial reagents. The resistance or sensitivity patterns can be used to help identify
anaerobic cocci. In particular, P. anaerobius can be readily picked out by it’s sensitivity to SPS and P. magnus can be distinguished
from P. micros by its resistance to Novobiocin.
Page 4 of 6
Procedure
1. Spread sample of test organism onto a plate containing anaerobic agar.
2. Using sterile forceps, carefully place Microring
®
AC in the center of the plate.
Press each tip gently to ensure contact with surface.
3. Incubate overnight at 37° C under anaerobic conditions.
4. Following incubation, growth patterns around each tip are read
and used to identify the organism.
Code
MZ
SPS
NO
Color
White
Pink
Blue
Antibiotic
Metronidazole
Sodium Polyanethol
Sulphonate
Novobiocin
Amount
5ug
1mg
5ug
Microring
®
AC Identification
Species
Peptococcus niger
Peptostreptococcus asaccharolyticus
Peptostreptococcus indolicus
Peptostreptococcus magnus
Anaerococcus prevotil
Peptostreptococcus anaerobius
Peptostreptococcus micros
Peptostreptococcus productus
Peptostreptococcus tetradius
Atopbium parvulum
Staphylococcus saccharolyticus
Other microaerophilic streptococci
Streptococcus intermedius
Gemella morbillorum
Streptococcus constellatus
R
R
R
S
S
S
R
R
R
-
-
-
L,a
L,a
L,a
Metronidazole
S
S
S
S
S
S
S
S
S
S
R/S
S
R
Novobiocin
R
R
R
R
R
S
S
S
SPS
R
R
R
R
R
S
R
R
R
R
R
Indole
-
+
+
-
-
-
-
-
-
-
-
Fatty Acids from PYG broth*
B,C,iv,a
B,(A,p,l)
B,(A,p,l)
A
B,A,(L),p
IC,A,(iv,ib,b)
A(s)
A,l,s
L,B(A,p)
L,a
F/A
S = Suseptibility, R = Resistant, R/S = aerotolerant strains resistant
(a) Occasional strains may give a zone size >12mm, but these organisms are easily distinguishable from
Peptostreptococcus anaerobius
as they are resistant to Novobicin.
(b) Occasional strains may give a small zone to SPS < 12mm in diameter.
_
* Gas-liquid chromotography analysis of fatty acid products in Peptone Yeast extract Glucose (PYG) broth.
Capital letters indicate major metabolic products, small letters indicate minor products and parentheses indicate variable reaction.
Storage & Handling
Store Microring
®
AN at 2-8°C
Rings maintain a shelf life of 12 months from manufacture date.
Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information
given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since
conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice
any process in violation of any law or any government regulation.
GV is a filter paper ring used for identification of Gardnerella vaginalis. The ring comes complete with 4 individual printed
tips containing various antimicrobial reagents. Each tip of the ring contains one of the following reagents; MTZ (metronidazole), TRM
(trimethoprim), STZ (sulphathiazole), and BIL, (bile salt). Each tip performs as a single identification disc because of an isolating
hydrophobic barrier. On blood agar, Gardnerella is identified by sensitivity to 3 designated tips and resistance to 1 tip.
Procedure
1. Spread sample of test organism onto a plate containing Columbia Agar with blood.
2. Using sterile forceps, carefully place Microring
®
GV in the center of the plate. Press each tip gently to ensure contact with surface.
3. Incubate plate for 48 hours at 37° C in humid atmosphere containing 5% CO2.
4. Following incubation, growth patterns around each tip are read and used to identify the organism.
Species
Gardnerella Vaginalis
Vaginal Lactobacilli
Cat -ve Corynebacter
Mtz
S
R
R
Trm
S
R
R
Stz
R
V
V
Bil
S
R
R
Bile 5mg
Metronidazole
50 mcg
MTZ = metronidazole, TRM = trimethoprim, STZ = sulphathiazole, BIL = bile salt
S = Susceptible, R = Resistant, V = Variable
Storage & Handling
Store at 4° C, avoid prolonged exposure to direct sunlight.
Microring
®
GV should be handled with forceps.
Avoid contact with skin to prevent contamination.
Rings maintain a shelf life 12 of months from manufacture date.
Sulphathiazole
1mg
Trimethoprim
5 mcg
Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information
given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since
conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice
any process in violation of any law or any government regulation.
京公网安备 11010802033920号
Copyright © 2005-2026 EEWORLD.com.cn, Inc. All rights reserved
电子工程世界
