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SC-93381

器件型号:SC-93381
文件大小:670.79KB,共1页
厂商名称:SCBT
厂商官网:http://www.scbt.com/
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metallothionein 1F sirna

SC-93381器件文档内容

SANTA CRUZ BIOTECHNOLOGY, INC.

Metallothionein 1F siRNA (h): sc-93381

                                                                                  The Power to Question

BACKGROUND                                                                        STORAGE AND RESUSPENSION

Metallothionein (MT) is a sulfhydryl- and cysteine-rich protein found in micro-   Store lyophilized siRNA duplex at -20 C with desiccant. Stable for at least
organisms, plants and all invertebrate and vertebrate animals. Metallothi-        one year from the date of shipment. Once resuspended, store at -20 C,
oneins are a group of ubiquitous low-molecular-weight proteins that have          avoid contact with RNases and repeated freeze thaw cycles.
functional roles in cell growth, repair and differentiation. They are implicated
primarily in metal ion detoxification, in that they are essential for the         Resuspend lyophilized siRNA duplex in 330 l of the RNAse-free water
protection of cells against the toxicity of cadmium, mercury and copper.          provided. Resuspension of the siRNA duplex in 330 l of RNAse-free water
Metallothionein, as an acute phase or stress-response protein and free radical    makes a 10 M solution in a 10 M Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM
scavenger, is related to inflammation and cellular protection from reactive       EDTA buffered solution.
forms of oxygen, ionizing radiation, pharmacological agents and mutagens.
Metallothioneins are known to be broadly expressed in heart, liver, kidney,       APPLICATIONS
breast and testis tissue.
                                                                                  Metallothionein 1F siRNA (h) is recommended for the inhibition of
REFERENCES                                                                        Metallothionein 1F expression in human cells.

1. Ioachim, E.E., et al. 2000. Immunohistochemical localization of                SUPPORT REAGENTS
  Metallothionein in endometrial lesions. J. Pathol. 191: 269-273.
                                                                                  For optimal siRNA transfection efficiency, Santa Cruz Biotechnology's
2. Liu, J., et al. 2000. Metallothionein-I/II null mice are more sensitive than   siRNA Transfection Reagent: sc-29528 (0.3 ml), siRNA Transfection
  wild-type mice to the hepatotoxic and nephrotoxic effects of chronic oral       Medium: sc-36868 (20 ml) and siRNA Dilution Buffer: sc-29527 (1.5 ml)
  or injected inorganic arsenicals. Toxicol. Sci. 55: 460-467.                    are recommended. Control siRNAs or Fluorescein Conjugated Control
                                                                                  siRNAs are available as 10M in 60 l. Each contain a scrambled sequence
3. Cai, L., et al. 2000. Induction of Metallothionein synthesis with preserva-    that will not lead to the specific degradation of any known cellular mRNA.
  tion of testicular function in rats following long term renal transplantation.  Fluorescein Conjugated Control siRNAs include: sc-36869, sc-44239,
  Urol. Res. 28: 97-103.                                                          sc-44240 and sc-44241. Control siRNAs include: sc-37007, sc-44230,
                                                                                  sc-44231, sc-44232, sc-44233, sc-44234, sc-44235, sc-44236, sc-44237
4. Florianczyk, B., et al. 2000. Metallothionein levels in cell fractions from    and sc-44238. RT-PCR Primer is not available for this product.
  breast cancer tissues. Acta Oncol. 39: 141-143.

5. Theocharis, S.E., et al. 2000. Liver Metallothionein expression in thioa-
  cetamide-intoxicated rats. Pathol. Res. Pract. 196: 313-319.

6. Kang, Y.J., et al. 2000. Metallothionein inhibits myocardial apoptosis in
  copper-deficient mice: role of atrial natriuretic peptide. Lab. Invest. 80:
  745-757.

7. Syring, R.A., et al. 2000. Cloning and sequencing of cDNAs encoding
  for a novel copper-specific Metallothionein and two cadmium-inducible
  Metallothioneins from the blue crab Callinectes sapidus. Comp. Biochem.
  Physiol. C Toxicol. Pharmacol. 125: 325-332.

CHROMOSOMAL LOCATION
Genetic locus: MT1F (human) mapping to 16q13.

PRODUCT

Metallothionein 1F siRNA (h) is a target-specific 20-25 nt siRNA designed to
knock down gene expression. Each vial contains 3 nmol of lyophilized siRNA,
sufficient for a 10 M solution once resuspended using protocol below.
Suitable for 50-100 transfections.

RESEARCH USE
For research use only, not for use in diagnostic procedures.

PROTOCOLS

See our web site at www.scbt.com or our catalog for detailed protocols and
support products.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com
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