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SC-81632

器件型号:SC-81632
文件大小:694.28KB,共1页
厂商名称:SCBT
厂商官网:http://www.scbt.com/
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器件描述

integrin ??V/??5

SC-81632器件文档内容

SANTA CRUZ BIOTECHNOLOGY, INC.

Integrin V/5 (P1F6): sc-81632

                                                                                 The Power to Question

BACKGROUND                                                                       APPLICATIONS
                                                                                 Integrin V/5 (P1F6) is recommended for detection of Integrin V/5 of
Integrins are heterodimers composed of noncovalently associated transmem-        human origin by immunoprecipitation [12 g per 100500 g of total protein
brane  and  subunits. The 16  and 8  subunits heterodimerize to produce          (1 ml of cell lysate)] and immunofluorescence (starting dilution 1:50, dilution
more than 20 different receptors. Most integrin receptors bind ligands that      range 1:50-1:500).
are components of the extracellular matrix, including Fibronectin, collagen
and Vitronectin. Certain integrins can also bind to soluble ligands such as      Suitable for use as control antibody for Integrin V siRNA (h): sc-29373.
Fibrinogen, or to counter receptors on adjacent cells such as the intracellular
adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to    Positive Controls: THP-1 cell lysate: sc-2238
cross-link or cluster integrins by binding to adjacent integrin receptors; both
receptor clustering and ligand occupancy are necessary for the activation of     RECOMMENDED SECONDARY REAGENTS
integrin-mediated responses. In addition to mediating cell adhesion and          To ensure optimal results, the following support (secondary) reagents are
cytoskeletal organization, integrins function as signaling receptors. Signals    recommended: 1) Immunoprecipitation: use Protein A/G PLUS-Agarose:
transduced by integrins play a role in many biological processes, including      sc-2003 (0.5 ml agarose/2.0 ml). 2) Immunofluorescence: use goat anti-
cell growth, differentiation, migration and apoptosis.                           mouse IgG-FITC: sc-2010 (dilution range: 1:100-1:400) or goat anti-mouse
                                                                                 IgG-TR: sc-2781 (dilution range: 1:100-1:400) with UltraCruzTM Mounting
REFERENCES                                                                       Medium: sc-24941.

1. Hynes, R.O. 1992. Integrins: versatility, modulation, and signaling in cell   PROTOCOLS
  adhesion. Cell 69: 11-25.                                                      See our web site at www.scbt.com or our catalog for detailed protocols
                                                                                 and support products.
2. Miyamoto, S., Akiyama, S.K. and Yamada, K.M. 1995. Synergistic roles for
  receptor occupancy and aggregation in integrin transmembrane function.
  Science 267: 883-885.

3. Clark, E.A. and Brugge, J.S. 1995. Integrins and signal transduction path-
  ways: the road taken. Science 268: 233-239.

4. Sheppard, D. 1996. Epithelial integrins. BioEssays 18: 655-660.

5. Juliano, R. 1996. Cooperation between soluble factors and integrin-
  mediated cell anchorage in the control of cell growth and differentiation.
  BioEssays 18: 911-917.

CHROMOSOMAL LOCATION

Genetic locus: ITGAV (human) mapping to 2q31-q32 and ITGB5 (human)
mapping to 3q21.2.

SOURCE

Integrin V/5 (P1F6) is a mouse monoclonal antibody raised against
UCLA P3 cells of human origin.

PRODUCT
Each vial contains 200 g IgG1 in 1.0 ml of PBS with < 0.1% sodium azide
and 0.1% gelatin.

Available azide-free for inhibition, sc-81632 L, 200 g/0.1 ml.

STORAGE

Store at 4 C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.

RESEARCH USE
For research use only, not for use in diagnostic procedures.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com
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