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SC-80191

器件型号:SC-80191
文件大小:687.98KB,共1页
厂商名称:SCBT
厂商官网:http://www.scbt.com/
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mif

SC-80191器件文档内容

SANTA CRUZ BIOTECHNOLOGY, INC.

MIF (Q18): sc-80191

                                                                                   The Power to Question

BACKGROUND                                                                         CHROMOSOMAL LOCATION
                                                                                   Genetic locus: MIF (human) mapping to 22q11.23.
Macrophage migration inhibitory factor, known as MIF or glycosylation-inhib-
iting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that          SOURCE
modulates macrophage and T cell function and is an important regulator             MIF (Q18) is a mouse monoclonal antibody raised against full length
of host response to infection. MIF is expressed at sites of inflammation,          recombinant MIF of human origin.
which suggests that it plays a role in regulating macrophage function in host
defense. MIF is produced by the pituitary gland and is found in monocytes,         PRODUCT
macrophages, differentiating immunological cells in the eye lens and brain,        Each vial contains 100 g IgG1 in 1.0 ml PBS with < 0.1% sodium azide and
and fibroblasts. Elevated levels of MIF protein are detected in the plasma         protein stabilizer.
of patients with severe sepsis or septic shock, a condition where MIF influ-
ences endotoxic shock by enhancing the production of other inflammatory            APPLICATIONS
cytokines including tumor necrosis factor  (TNF), interleukin-1 (IL-1) and         MIF (Q18) is recommended for detection of MIF of human origin by Western
interferon- (IFN-). MIF promotes the systemic inflammatory response by             Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipi-
counter-regulating glucocorticoid-mediated inhibition of immune-cell acti-         tation [1-2 g per 100-500 g of total protein (1 ml of cell lysate)] and solid
vation and proinflammatory cytokine production. MIF may mediate tissue             phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000).
destruction through the induction of proteinases.
                                                                                   Suitable for use as control antibody for MIF siRNA (h): sc-37137, MIF shRNA
REFERENCES                                                                         Plasmid (h): sc-37137-SH and MIF shRNA (h)

1. Weiser, W.Y., et al. 1989. Molecular cloning of a cDNA encoding a human         Lentiviral Particles: sc-37137-V.
  macrophage migration inhibitory factor. Proc. Natl. Acad. Sci. USA 86:
  7522-7526.                                                                       Molecular Weight of MIF: 12.5 kDa.

2. Paralkar, V., et al. 1994. Cloning the human gene for macrophage migration      Positive Controls: HL-60 whole cell lysate: sc-2209, Jurkat whole cell
  inhibitory factor (MIF). Genomics 19: 48-51.                                     lysate: sc-2204 or AML-193 whole cell lysate.

3. Bernhagen, J., et al. 1994. Purification, bioactivity, and secondary structure  RECOMMENDED SECONDARY REAGENTS
  analysis of mouse and human macrophage migration inhibitory factor (MIF).        To ensure optimal results, the following support (secondary) reagents are
  Biochemistry 33: 14144-14155.                                                    recommended: 1) Western Blotting: use goat anti-mouse IgG-HRP: sc-2005
                                                                                   (dilution range: 1:2000-1:32,000) or Cruz MarkerTM compatible goat anti-
4. Lubetsky, J.B., et al. 1999. Pro-1 of macrophage migration inhibitory factor    mouse IgG-HRP: sc-2031 (dilution range: 1:2000-1:5000), Cruz MarkerTM
  functions as a catalytic base in the phenylpyruvate tautomerase activity.        Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:
  Biochemistry 38: 7346-7354                                                       sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecip-
                                                                                   itation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml).
5. Onodera, S., et al. 1999. High expression of macrophage migration
  inhibitory factor in the synovial tissues of rheumatoid joints. Cytokine
  11: 163-167.

6. Benigni, F., et al. 2000. The proinflammatory mediator macrophage migra-
  tion inhibitory factor induces glucose catabolism in muscle. J. Clin. Invest.
  106: 1291-1300.

7. Calandra, T., et al. 2000. Protection from septic shock by neutralization of
  macrophage migration inhibitory factor. Nat. Med. 6: 164-170.

STORAGE

Store at 4 C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.

RESEARCH USE
For research use only, not for use in diagnostic procedures.

PROTOCOLS

See our web site at www.scbt.com or our catalog for detailed protocols and
support products.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com
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