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SC-42258

器件型号:SC-42258
文件大小:673.63KB,共1页
厂商名称:SCBT
厂商官网:http://www.scbt.com/
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slit2 sirna

SC-42258器件文档内容

SANTA CRUZ BIOTECHNOLOGY, INC.

Slit2 siRNA (h): sc-42258

                                                                                                                                                 The Power to Question

BACKGROUND                                                                           STORAGE AND RESUSPENSION

Secreted leucine-rich repeat-containing proteins 1-3 (Slit1-3) are secreted          Store lyophilized siRNA duplex at -20° C with desiccant. Stable for at least

glycoproteins that influence axonal guidance and mediate normal neural               one year from the date of shipment. Once resuspended, store at -20° C,

development by acting as high-affinity signaling ligands for the repulsive           avoid contact with RNases and repeated freeze thaw cycles.

guidance receptor, Roundabout (Robo). Within the developing central nervous          Resuspend lyophilized siRNA duplex in 330 µl of the RNAse-free water

system (CNS) of different vertebrate systems, Slit proteins are expressed in         provided. Resuspension of the siRNA duplex in 330 µl of RNAse-free water

equivalent regions, suggesting a conserved function among vertebrate                 makes a 10 µM solution in a 10 µM Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM

homologs. Slit is expressed in the midline of the central nervous system in          EDTA buffered solution.

both vertebrates and invertebrates, where it functions as a regulatory factor

of mesodermal cell movement during gastrulation. Slit2 is a short range              APPLICATIONS

inhibitory guidance cue for retinal ganglion cell (RGC) axons that may mediate

spatial progression of RGCs.                                                         Slit2 siRNA (h) is recommended for the inhibition of Slit2 expression in

                                                                                     human cells.

REFERENCES                                                                           Slit2 (E-20): sc-16619 is recommended as a control antibody for Western

1. Rothberg, J.M., et al. 1990. slit: an extracellular protein necessary for         Blotting (starting dilution 1:100, dilution range 1:100-1:1,000) or immuno-

development of midline glia and commissural axon pathways contains                   fluorescence (starting dilution 1:50, dilution range 1:50-1:500) protein

both EGF and LRR domains. Genes Dev. 4: 2169-2187.                                   detection using the recommended secondary reagents listed below.

2. Holmes, G.P., et al. 1998. Distinct but overlapping expression patterns of        RECOMMENDED SECONDARY REAGENTS

two vertebrate slit homologs implies functional roles in CNS development

and organogenesis. Mech. Dev. 79: 57-72.                                             To ensure optimal results, the following support (secondary) reagents are

3. Brose, K., et al. 1999. Slit proteins bind Robo receptors and have an evolu-      recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020

tionarily conserved role in repulsive axon guidance. Cell 96: 795-806.               (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey

                                                                                     anti-goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™

4. Hu, H. 1999. Chemorepulsion of neuronal migration by Slit2 in the devel-          Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:

oping mammalian forebrain. Neuron 23: 703-711.                                       sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo-

5. Yuan, W., et al. 1999. The mouse SLIT family: secreted ligands for ROBO           rescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100-

expressed in patterns that suggest a role in morphogenesis and axon                  1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400)

guidance. Dev. Biol. 212: 290-306.                                                   with UltraCruz™ Mounting Medium: sc-24941.

6. Erskine, L., et al. 2000. Retinal ganglion cell axon guidance in the mouse        SUPPORT REAGENTS

optic chiasm: expression and function of robos and slits. J. Neurosci. 20:           For optimal siRNA transfection efficiency, Santa Cruz Biotechnology’s

4975-4982.                                                                           siRNA Transfection Reagent: sc-29528 (0.3 ml), siRNA Transfection

7. Niclou, S.P., et al. 2000. Slit2 is a repellent for retinal ganglion cell axons.  Medium: sc-36868 (20 ml) and siRNA Dilution Buffer: sc-29527 (1.5 ml)

J. Neurosci. 20: 4962-4974.                                                          are recommended. Control siRNAs or Fluorescein Conjugated Control

                                                                                     siRNAs are available as 10µM in 60 µl. Each contain a scrambled sequence

CHROMOSOMAL LOCATION                                                                 that will not lead to the specific degradation of any known cellular mRNA.

Genetic locus: SLIT2 (human) mapping to 4p15.2; Slit2 (mouse) mapping to             Fluorescein Conjugated Control siRNAs include: sc-36869, sc-44239,

5 B3.                                                                                sc-44240 and sc-44241. Control siRNAs include: sc-37007, sc-44230,

                                                                                     sc-44231, sc-44232, sc-44233, sc-44234, sc-44235, sc-44236, sc-44237 and

PRODUCT                                                                              sc-44238. Semi-quantitative RT-PCR may be performed using RT-PCR

                                                                                     Primer: Slit2 (h)-PR: sc-42258-PR (20 µl, 464 bp). Annealing temperature

Slit2 siRNA (h) is a target-specific 20-25 nt siRNA designed to knock down           for the primers should be 55-60° C and the extension temperature should

gene expression. Each vial contains 3 nmoles of lyophilized siRNA, sufficient        be 68-72° C.

for a 10 µM solution once resuspended using protocol below. Suitable for

50-100 transfections.

RESEARCH USE

For research use only, not for use in diagnostic procedures.

PROTOCOLS

See our web site at www.scbt.com or our catalog for detailed protocols and

support products.

       Santa Cruz Biotechnology, Inc.     1.800.457.3801      831.457.3800     fax 831.457.3801  Europe  +00800 4573 8000  49 6221 4503 0  www.scbt.com
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